Dairy products help to meet the need of essential nutrients that are difficult to obtain in daily life without milk and its products like yogurt, cheese, butter. Milk is almost sterile when secreted from a healthy udder. Lower temperature and boiling the milk help in retarding the growth of spoilage organisms. Biopreservation is a method in which natural microbiota or antimicrobials are used in food to enhance the shelf-life. For this purpose affordable microorganisms are selected to control the pathogenic activity. The main organism which is used is lactic acid bacteria and their metabolites. They have the ability to show the antimicrobial features and maintain the sole flavor of food. Lactic acid bacteria are considered as great biopreservatives. Other LAB such as nisin has broad range of application in food industry and approved by food and drug administration (FDA). Other metabolites such as enterococci, Bacteriophages and endolysins used as bio-preservation and have promising role in milk storage. As these are economically important due to their drastic advantages like non-toxic, availability, non-immunogenic and broad activity so, they are considered to be good agent for biopreservation. This review will focus on application of biopreservatives to reduce the spoilage and increase the safety of milk products.

Keywords: Bacteriocins, Lactic Acid Bacteria (LAB), Enterococci, Milk, Cheese, Cream.

S. E. Evivie, G. C. Huo, J. O. Igene, and X. Bian, “Some current applications, limitations and future perspectives of lactic acid bacteria as probiotics,” Food Nutr. Res., vol. 61, no. 1, 2017.

M. Rai, R. Pandit, S. Gaikwad, and G. Kövics, “Antimicrobial peptides as natural bio-preservative to enhance the shelf-life of food,” J. Food Sci. Technol., vol. 53, no. 9, pp. 3381–3394, 2016.

M. Leyva Salas, J. Mounier, F. Valence, M. Coton, A. Thierry, and E. Coton, “Antifungal Microbial Agents for Food Biopreservation—A Review,” Microorganisms, vol. 5, no. 3, p. 37, 2017.

T. K. Thorning, A. Raben, T. Tholstrup, S. S. Soedamah-muthu, I. Givens, and A. Astrup, “Milk and dairy science,” vol. 1, pp. 1–11, 2016.

J. D. Murray, N. Mohamad-Fauzi, C. A. Cooper, and E. A. Maga, “Current status of transgenic animal research for human health applications".

D. E. Kerr and O. Wellnitz, “Mammary expression of new genes to combat mastitis,” J. Anim. Sci., vol. 81, no. 15_suppl_3, pp. 38–47, Mar. 2003.

D. M. Donovan, D. E. Kerr, and R. J. Wall, “Engineering disease resistant cattle,” Transgenic Res., vol. 14, no. 5, pp. 563–567, 2005.

D. E. Kerr et al., “Lysostaphin expression in mammary glands confers protection against staphylococcal infection in transgenic mice,” Nat. Biotechnol., vol. 19, no. 1, pp. 66–70, 2001.

L. M. Houdebine, “Transgenic animal production,” Biotechnol. Sustain. Agric. Emerg. Approaches Strateg., vol. 61, pp. 141–184, 2017.

S. Mishra et al., “Effects of nutraceuticals on genetic expressions.,” Open Nutraceuticals J., vol. 2, pp. 70–80, 2009.

L. Luo et al., “Fabrication of Synthetic Mesenchymal Stem Cells for the Treatment of Acute Myocardial Infarction in Mice,” Circ. Res., vol. 120, no. 11, pp. 1768–1775, 2017.

D. R. Brundige, E. A. Maga, K. C. Klasing, and J. D. Murray, “Lysozyme Transgenic Goats’ Milk Influences Gastrointestinal Morphology in Young Pigs,” J. Nutr., vol. 138, no. 5, pp. 921–926, 2018.

Rai, M., Pandit, R., Gaikwad, S. & Kövics, G. (2016). Antimicrobial peptides as natural bio-preservatives to enhance the shelf-life of food. Journal of Food Science and Technology, 53, 3381–3394.

Singh, V.P. (2018). Recent approaches to food bio-preservation – a review. Open Veterinary Journal, 8, 104.

Arqués, J.L., Rodríguez, E., Langa, S., Landete, J.M. & Medina, M. (2015). Antimicrobial activity of lactic acid bacteria in dairy products and gut: Effect on pathogens. BioMed Research International, 2015.

Bhardwaj, A., Malik, R.K. & Chauhan, P. (2008). Functional and safety aspects of enterococci in dairy foods. Indian Journal of Microbiology, 48, 317–325.

Gautam, N. & Sharma, N. (2009). Bacteriocin: Safest approach to preserve food products. Indian Journal of Microbiology, 49, 204–211.

Silva, C.C.G., Silva, S.P.M. & Ribeiro, S.C. (2018). Application of bacteriocins and protective  cultures in dairy food preservation. Frontiers in Microbiology, 9.

Leyva Salas, Morphology in Young Pigs. The Journal of Nutrition 138:921–926. doi: 10.1093/jn/138.5.921.

Donovan DM, Kerr DE, Wall RJ (2005) Engineering disease resistant cattle. Transgenic Research 14:563–567. doi: 10.1007/s11248-005-0670-8.

Hickey, C.D., Sheehan, J.J., Wilkinson, M.G. & Auty, M.A.E. (2015). Growth and location of bacterial colonies within dairy foods using microscopy techniques: A review. Frontiers in Microbiology, 6, 1–8.

Hill, D., Sugrue, I., Arendt, E., Hill, C., Stanton, C. & Ross, R.P. (2017). Recent advances in microbial fermentation for dairy and health. F1000Research, 6, 751.