Pearl millet flour was fortified with Bambara groundnut flour (0-20%) to form blends used in Kunun tsamiya preparation. Functional and microbiological profiles of the flours and modified kunu samples as well as proximate and sensory properties were evaluated. Fortification had raised the nutritional value of the foods. Swelling power and water absorption capacity had increased with an increase in the level of bambara groundnut flour addition whereas bulk density and viscosity had decreased, the results were as follows- swelling power (23.512-27.654%), water absorption capacity (3.300-4.650 g/g), bulk density (0.791-0.779 g/cm3), viscosity (48.625-56.555 mPa-s). The moisture content, ash, fat, protein, fiber, carbohydrate and total energy levels were found as follows- 78.90-83.64%, 0.24-0.35%, 1.88-3.95%, 3.52-6.02%, 0.70-0.78%, 5.27-14.77% and 80.69-90.02 kcal/100g respectively. The concentration of anti-nutritional factors, alkaloids, flavonoids, phytic acids and tannins ranged from 0.03 to 0.07g/100g, 0.12 to 0.19g/100g, 2.21 to 4.29g/100g and 0.04 to 0.07g/100g respectively. The minerals contents were found to be significant as follows Ca (21.82 to 26.53 mg/100g), Fe (5.19 to 6.24 mg/100g), Zn (1.71 to 2.11 mg/100g), P (83.39 to 93.28 mg/100g), Mg (1.04 to 1.08 mg/100g), K (65.84 to 81.34 mg/100g), Na (8.60 to 10.59 mg/100g). In-vitro protein digestibility at 1h ranged between 66.86 to 68.76% and 68.17 to 69.17% at 6hrs; starch digestibility ranged between 68.99 and 72.17%. Microbial findings generally revealed moderate counts with a total microbial load of 1.41x103cfu/g in the K0MfB (100:00) formulation. Salmonella and Escherichia coli were not detected in Kunun tsamiya samples made from an 80:20% millet-bambara nut flour blend. Staphylococcus aureus, Staphylococcus epidemidis, Salmonella spp, Klebsiella spp, Pseudomonas spp and Escherichia coli were isolated with the following percentage occurrences 27.92%, 20.13%, 12.34%, 16.88%, 17.86% and 4.87% respectively. Sensory evaluation results revealed that Kunun tsamiya from 80:20% millet-bambara groundnut nut flour blends had better taste, aroma and general acceptability and as such the food formulation was the most liked, nutritious and as well most preferred by the test panelists. Therefore it's concluded that fortification of pearl millet with bambara groundnut at 20% level enhanced the nutrient density of the resulting kunu tsamiya without adversely affecting its well-known organoleptic properties.
Keywords: Pearl millet, Bambara groundnut, Supplementation, Kunun tsamiya, Invitro-digestibility, Antinutrients, Minerals, Bacteria prevalence, Functional properties, Food fortification.
Adebayo-Oyetoro, A.O., Shotunde, A.B., Adeyeye Samuel, A.O., & Ogundipe, O.O. (2017). Quality Evaluation of Millet-Based Fura Powder Supplemented with Bambara Groundnut. International Journal of Food Science, Nutrition and Dietetics, 6(3): 358–362.
Adu-Dapaah, H.K., & Sangwan, R.S. (2004). Improving Bambara Groundnut Productivity using gamma Irradiation and in-vitro techniques. African Journal of Biotechnology, 3: 260–265.
Agbara, G.I., Masaya, F.A., Badau, M.H., Igwegbe, O.A., & Yakaka, A.M. (2018). Evaluation of Functional, Proximate, Microbial and Sensory Properties of Pearl Millet-Bambara Groundnut Yartsala (A Traditional Deep- Fried Fermented Puff). Asian Journal of Agriculture and Food Sciences, 6(2).
Ali, M.A.M., Tinay, A.H., & Abdalla, A.H. (2003). Effect of Fermentation on the in-vitro Protein Digestibility of Pearl Millet. Food Chemistry, 80(1): 51–54.
Amarteifio, J.O., Tibe, O., & Njogu, R.M. (2006). The Mineral Composition of Bambara Groundnut (Vigna subterranea (L) Verdc) grown in Southern Africa. African Journal of Biotechnology, 5(23): 2408–2411.
Annor, G.A., Tyl, C., Marcone, M., Ragee, S., & Marti, A. (2017). Why do Millets have lower Starch and Protein Digestibility than other Cereals? Trends in Food Science and Technology, 66(C): 78–83.
APHA (1992). Compendium of Methods for Microbiological Examination of Foods 3rd Editio. American Public Health Association.
AOAC (1990). Official Methods of Analysis (15th edition). Arlington, VA: Association of Official Analytical Chemists.
AOAC (2006). Official Methods of Analysis (18th edition). Association of Official Analytical Chemists.
Aremu, M.O., Ibrahim, H., & Ekanem, B. (2016). Effect of Processing on In-vitro Protein Digestibility and Anti-Nutritional Properties of three under-utilized Legumes Grown in Nigeria. BBJ/2258.
Ayo, J.A., & Aba, F. (2020). Effect of Bambara Groundnut Flour Addition on Proximate, Mineral Composition and Sensory Quality of Millet-Based Madidi: A Nigerian Solid Gel Food. Asian Journal of Food Science,16(4): 1–8.
Baltensperger, D.D. (1996). Foxtail and Proso Millet in: Progress in New Crops. Progress in New Crops, ASHS Press, Alexandria, Pages 182–190.
Center for Food Safety (2007). Microbiological Guidelines for Ready-to-eat Foods. Centre for Food Safety and Environmental Hygiene Department, 66 Queensway, Hong Kong.
Chinma, C.E., & Gernah, D.I. (2007). Physicochemical and Sensory Properties of Cookies Produced from Cassava/Soybean/Mango Composite Flour. Journal of Food Technology, 5(3): 256–260.
Chausmer, A.B. (1998). Zinc, Insulin and Diebeties. Journal of the American College of Nutrition, 17(2): 109–115.
El-Hag, M.E., El-Tinay, A.H., & Yousif, N.E. (2002). Effect of fermentation and De-hulling on Starch, total Polyphenols, Phytic Acid Content and in-vitro Protein Digestibility of Pearl Millet. Food Chemistry, 77: 193–196.
Falegan, C.R., Akoja, S.O., & Oyarekua, M.A. (2017). Microbiological Assessment of Suya in Ado Ekiti Metropolis, Ekiti State, Nigeria. MOJ Biol. Med., 2(3): 266–269.
FAO (2002). Food and Agriculture Organization/World Health Organization Expert Consultation. Magnesium. In Human Vitamin and Mineral Requirements, Rome and Geneva: Food and Agriculture Organization of the United Nations and World Health Organization.
FAO, ICRISAT (1996). The world Sorghum and Millet Economics. Facts Trends and Outlook. Food and Agriculture of the United Nations, Rome, Italy and International crops Research Institute for the Semi-arid Tropics, Pantacharu 502324, India.
FAO (2008). Sorghum and Millets in Human Nutrition. FAO Food and Nutrition Series, No. 27, United Nations Food and Agriculture Organization, Rome.
Food Standards, Australia New Zealand (2002). Food Standards Code, Vol.2. Canberra: Information Australia.
Fasoyiro, S.B, Ajibade, S.R., Omole, A.J., Adeniyan, O.N., & Farinde, E.O. (2006). Proximate, Minerals and Anti- nutritional factors of some Underutilized Grain Legumes in South-Western Nigeria. Nutrition Food Science, 36: 18–23.
Florence, S.P., Asna, U., Asha, M.R., & Jyotsna, R. (2014). Sensory, Physical and Nutritional Qualities of Cookies Prepared from Pearl Millet (Pennisetum typhoideum). Journal of Food Processing and Technology, 5(10): 377.
Gilbert, U., & Harrison A. (2001). Occurrence of enterotoxin producing Staphylococcus aureus in meat market in Nigeria. Journal of Food Infection, 56: 25–35.
Goli, A.E. (1997). Bambara Groundnut (Vigna subterranean (L) Verdc.). In Heller, J., Begemann, F., Mushonda, J. (Eds.), Promoting the Conservation and use of Underutilized and Neglected Crops, International Plant Genetic Resources Institute, Rome.
Gomez, K.A., & Gomez, A.H. (1984). Statistical Procedures for Agricultural Research, second edition. Wiley and sons Inc., New York, USA.
Gull, A., Prasad, K., & Kumar, P. (2015). Effect of Millet Flours and Carrot Pomace on Cooking Qualities, Colour and Texture of Developed Pasta. Food Science and Technology, 63: 470–474.
Griffths, D.W. (1985). The Inhibition of Digestive Enzymes by Polyphenolic Compounds. Exp. Biologically. Mediated., 199: 504–516.
Hillocks, R.J., Bennett, C., & Mponda, O.M. (2012). Bambara Groundnut: A Review of Utilisation, Market Potential and Crop Improvement. African Crop Science Journal, 20(1): 1–16.
Igbabul, B., Adole, D., & Sule, S. (2013). Proximate Composition, Functional and Sensory Properties of Bambara groundnut (Voandzeia subterranean), Cassava (Manihot Esculentus) and Soybean (Glycine Max) Flour Blends for “Akpekpa” Production. Current Research Nutrition Food Science, 1(2).
Ihekoronye, A.I., & Ngoddy, P.O. (1985). Integrated Food Science and Technology for the Tropics. Macmillan Publisher Ltd. London, Pages 288–289.
James, S., Akosu N.I., Maina, Y.C., Baba, A.I., Nwokocha, L., Amuga S.J., Yohanna, A., & Maxwell Omeiza, Y.M. (2018). Effect of Addition of Processed Bambara Groundnut on the Functional and Sensory Acceptability of Millet-Based Infant Formula. Food Science Nutriition, 6(4): 783–790.
Jideani, V.A. (1999). Traditional and possible Technology uses of Digitaria exillis (Acha) and Digitaria ibrua. Review Plant Foods Human Nutrition, 54: 362–373.
Jideni, V.A, Nkama, I., & Agbo, E.D. (2001). Survey of Fura production in some North-Eastern States of Nigeria. Plant Food Human Nutrition, 56: 23–26.
Jones, M.J.,Tanya, V.N., Mbofung, C.M.F., Fonkem, D.N., & Silverside D.E. (2001). A Microbiological and Nutritional Evaluation of the West African Dried Meat Product. Kilishi Journal of Food Technology in Africa, 6: 126–129.
Karuna, D., Noel, G., & Dilip, K. (1996). Food and Nutrition Bulletin. United Nation University, 17(2): 252–253.
Kheterpaul, N., & Chauhan, B.M. (1991). Effect of Natural Fermentation on Phytate and Polyphenolic Content and in-vitro Digestibility of Starch and Protein of Pearl Fillet (P. typhodeum). Journal of Science, Food and Agriculture, 55: 189–195.
Kulthe, A.A., Thorat, S.S., & Lande, S.B. (2016). Characterization of Pearl Millet Cultivars for Proximate Composition, Minerals and Anti-nutritional Contents. Advances in Life Sciences, 5(11): 4672–4675.
Kumar, A., & Chauhan, B.M. (1993). Effects of phytic acid on protein digestibility (in vitro) and HCl- Extractability of Minerals in Pearl Millet sprouts. Cereal Chemistry, 70: 504–506.
Léder, I. (2004). Sorghum and Millets. In Cultivated Plants, Primarily as Food Sources, in Encyclopedia of Life Support Systems (EOLSS), Developed under the Auspices of the UNESCO, Eolss Publishers, Oxford, UK.
Malik, H., Gulzar, A.N., & Dar, B.N. (2015). Optimisation of Process for Development of Nutritionally Enriched Multigrain Bread. Journal of Food Process Technology, 7: 544.
Mkandawire, C.H. (2007). Review of Bambara groundnut production. Sub-Saharan Africa. Journal of Agriculture, 2: 464–470.
Mohammed Nour, A.A., Mohamed, A.R., Adiamo, O.Q., & Babiker, E.E. (2018). Changes in Protein Nutritional Quality as affected by Processing of Millet Supplemented with Moringa Seed Flour. Journal of the Saudi Society of Agricultural Sciences, 17(3): 275–281.
NHS Direct online (2017). www.england.nhs.uk/participation.
Nkama, I. (1993). Studies on Improving the Nutritional Quality of Masa-a Traditional Nigerian Fermented Cereal Based Food. A report to the United Nations University, Mysore, India, Central Food Technology Research Institute (CFTRI), Pages 1–28.
Nkama, I., & Ikwelle, M.C. (1997). Assessment of Food Quality of Millet Grain. Pearl-Millet in Nigerian Agriculture, Production, Utilization and Research Priorities. Proceeding of the Pre-season National Coordinated Research Program on Pearl Millet, Maiduguri.
Nkama, I., & Malleshi, N.G. (1998). Production and Evaluation of Sorghum and Pearl Millet Tech., Nutritional Quality of Traditional Masa form mixture of Manual No. 2 International Crop Institute for Semi Rice, Pearl Millet, Cowpea and Groundnut. Food Nutition Arid Crops (ICRISAT) Bull., 19: 336–373.
Obilana, A.B., & Manyasa, E. (2002). Millets. Pages 177-217 in Pseudo Cereals and Less Common Cereals: Grain Properties and Utilization Potential, Springer-Verlag, New York.
Ogbonna I.O., Danladi, M.S., Akinmusire O., & Odu C.E. (2012). Microbiological Safety and Proximate Composition of Suya stored at Ambient Temperature for six hours from Maiduguri, Northern Nigeria. Internet Journal of Food Safety, 14: 11–16.
Olaleye, A., Adeyeye, E., & Adesina, A. (2013). Chemical Composition of Bambara Groundnut (Vigna. subterranea L. Verdc). Seed parts. Bangladesh Journal of Scientific and Industrial Research, 48(3): 167–178.
Onwuka, G.I. (2005). Food Analysis and Instrumentation: Theory and Practice. Naphathali Prints, Nigeria.
Owheruo, J.O., Ifesan, B.O.T., & Kolawole, A.O. (2018). Physicochemical Properties of Malted Finger (Eleusine corocana) and Pearl Millet (Pennisetum glaucum). Journal of Food Science and Nutrition, 7: 476–482.
Plaami, S.P. (1997). Content of Dietary Fiber in Foods and its Physiological Effects. Food Rev. Int., 13: 27–76.
Reiman H.P., & Cliver D.O. (Eds.) (2006). Foodborne Infections International. Research Journal Pub. Environ. Health.
Rolfes, S.R., Pinna, K., & Whitney, E. (2009). Understanding normal and Clinical Nutrition (8th Edition). Nutrition and Diet Therapy, Delmar Learning, Thomson Learning Inc. Canada.
Ruth, S., Johanna, N., Disorn, S., Tobias, N., Mirja, E.G., Lena, R., Christopher, L.S., Stackelberg, O.V., Johnson, T., Nabers, D., Romy, K., Kratz, M., Hans-Ulrich, K., Cornelia, M.U., & Rudolf, K.T. (2018). Effects of Intermittent and Continuous Calorie Restriction on Body Weight and Metabolism over 50 wk: a Randomized Controlled Trial. The American Journal of Clinical Nutrition, 108(5): 933–945.
Taylor, J., & Taylor, J.R.N. (2002). Alleviation of the Adverse Effects of Cooking on Protein Digestibility in Sorghum through Fermentation in Traditional African Porridges. International Journal of Food Science and Technology, 37: 129–138.
Yagoub, A.E.G.A., & Abdalla, A.A. (2007). Effect of Domestic Processing Methods on Chemical Composition, In-vitro Digestibility of Protein and Starch and Functional Properties of Bambara Groundnut (Vigna subterranea) Seed Research. Journal of Agricultural Biology.
Source of Funding:
This study did not receive any grant from funding agencies in the public, commercial, or not-for-profit sectors.
Competing Interests Statement:
The authors declare having no competing interest with any party concerned during this publication.
Consent for publication:
The authors declare that they consented to the publication of this study.
Authors' contributions:
All the authors made full contribution starting from proposal writing, visualization, methodology, data analysis, first draft writing, review and editing.
A New Issue was published – Volume 7, Issue 4, 2024
09-10-2024 07-07-2024