Japanese encephalitis is a public health problem in many parts of Myanmar. The diseases are transmitted to human by the biting of vector mosquitoes. The study was conducted in JE suspected cases occurred villages ThaPhan Pinseit and ThaPyu Pinseit villages in ThaPhan Pinseit RHC and PaukGone and SarPhyusu villages in PaukGone village in PaukGone RHC from Ayeyawady Region were selected for JE vector surveillance from December 2023 to March 2024. 50 houses from the pigs were randomly selected from each village. Mosquitoes were collected in all selected houses and pig farms using WHO sucking tubes. Larvae were collected in and around 1 Kilometer radius from study sites. Result found that JE main vector Cx. tritaeniorhynchus was abundantly collected in all study village and the highest number of vectors Cx. tritaeniorhynchus was collected from SarPhyusu village 2284 followed by ThaPyu Pinseit 1890 and lowest number of main vectors was collected in ThaPhan Pinseit village (1268). In overall collected mosquitoes, the highest density was found 74.18% of main vector Cx. tritaeniorhynchus followed by suspected vector Cx. vishnui 10.04%, and lowest density was observed Mansonia and An. vagus 0.33% each. Peak biting time of main vector Cx. tritaeniorhynchus was found 9-10 pm. Suspected vector Cx. vishnui was also collected in high number in all villages. Nine species of mosquitoes were collected from the study villages. Of this only four species of An. hyrcanus, An. barbirostris, An. vagus and An. tesellatus were collected. These Anopheles species were available in PaukGone and SarPhyusu villages. Anopheles hyrcanus was collected from all villages. Culex tritaeniorhynchus larvae were abundantly collected from rice fields, polluted water pools and stagnant water pools with plenty of hyacinth plants and also larvae were collected in concrete jars, Bago jars and metal drums water which water was stored for fire water. High density of main vector and suspected vector were collected in all JE suspected villages, pig farms, pigs, and breeding sources of vectors mosquitoes were available in the villages and also JE suspected cases are available in the all villages. This study provides information of high collection of main vector adult and larvae on risk of further transmission in the study areas. Environmental and ecological factors are responsible for the spread of JE virus. Therefore, awareness of community to vector borne diseases control activities and health education to all family members should be strengthened to prevent JE transmission in the villages.

Keywords: Japanese Encephalitis, JE, Main vector, Cx. tritaeniorhynchus, Larvae, Habitats, Rice fields, Water pools, Pig farm, Density, Positivity.

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Source of Funding:

This study did not receive any grant from funding agencies in the public, commercial, or not-for-profit sectors.

Competing Interests Statement:

The authors declare no competing financial, professional, or personal interests.

Consent for publication:

The authors declare that they consented to the publication of this study.

Authors' contributions:

All the authors took part in literature review, analysis and manuscript writing equally.