Molecular markers are the DNA sequences that are used to tag or highlight specific genes or nucleotide sequence in the genome. In the past, diagnosis, and monitoring of infectious disease, there many conventional methods like Biotyping, Ribotyping and Protein analyses are used but these methods are not so reliable and exact. So by the invention of the molecular markers, the detection of diseases was started by using these molecular markers. In this review, we discussed different types of molecular markers that are used to detect diseases and many purposes as diagnostic tools. The Molecular markers include Restriction fragment length polymorphism (RFLP), Random amplification of polymorphic DNA (RAPD), Amplified fragment length polymorphism (AFLP), Simple sequence repeats (SSR), Inter-Simple Sequence Repeat Amplification (ISSR), Cleaved Amplified Polymorphic Sequences (CAPS) and Sequence Characterized Amplified Region (SCAR) are discussed in the article.

Keywords: RFLP, RAPD, AFLP, SSR, ISSR, CAPS.

Tang Y, Procop GW, Persing DH. Molecular diagnostics of infectious diseases. 1997;2038:2021-2038.

Khlestkina EK. Molecular Markers in Genetic Studies and Breeding. 2014;4(3):236-244. doi:10.1134/S2079059714030022.

Kordrostami M, Rahimi M. Molecular markers in plants : Concepts and applications. 2015; (September).

Hamburgo N, Alegre P, State S, Hamburgo N, Alegre P. Molecular techniques for the study and diagnosis of parasite infection. 2011;17(3):239-248.

Higuera AD La, Gutihez J, Likbana J, Garcia-mendoxa A. A new biotyping method for Streptococcus mutans with the API ZYM system. 1999:88-91.

Tenover FC, Arbeit R, Archer G, et al. Comparison of Traditional and Molecular Methods of Typing Isolates of Staphylococcus aureus. 1994;32(2):407-415.

Relander P. The Demonstration By Ribotyping Of The Stability Of Oral Streptococcus Mutans Infection Over 5 To 7 Years In Children. 1994;39(6):467-471.

Fernández-álvarez C, Torres-corral Y, Santos Y. SC. J Proteomics. 2017. doi:10.1016/j.jprot.2017.09.007.

Tharachand C, C IS, Mn M. Molecular markers in characterization of medicinal plants : An overview. 2012;2(2):1-12.

Finger A, Klank C. Review Molecular Methods : Blessing or Curse ? 2010:309-320. doi:10.1007/978-3-540-92160-8.

Bardakci F. Random Amplified Polymorphic DNA ( RAPD ) Markers. 2001;25:185-196.

Kumar NS, Gurusubramanian G. Random amplified polymorphic DNA ( RAPD ) markers and its applications. 2011;11(3):116-124.

Agarwal M, Shrivastava ÆN, Á SÁRÁP. Advances in molecular marker techniques and their applications in plant sciences. 2008:617-631. doi:10.1007/s00299-008-0507-z.

Lucia M, Vieira C, Santini L, Diniz AL, Munhoz CDF. Microsatellite markers : what they mean and why they are so useful. 2016;328:312-328.

Shehata AI, Al-ghethar HA, Al-homaidan AA. Application of simple sequence repeat ( SSR ) markers for molecular diversity and heterozygosity analysis in maize inbred lines. Saudi J Biol Sci. 2009;16(2):57-62. doi:10.1016/j.sjbs.2009.10.001.

Jonah PM, Bello LL, Lucky O, Midau A, Moruppa SM. Review : The Importance of Molecular Markers in. 2011;11(5).

Kesawat MS, Das BK. Molecular Markers : It ’ s Application in Crop Improvement. 2009;2009(10):169-181.

Adinolfi B, Chicca A, Martinotti E, Breschi MC, Nieri P. Sequence characterized amplified region (SCAR) analysis on DNA from the three medicinal Echinacea species. 2007;78:43-45. doi:10.1016/j.fitote.2006.09.012.

Molecular Markers in Crop Improvement Indian Institute of Pulses Research.

Jiang G. Molecular Markers and Marker-Assisted Breeding in Plants. 2010.

Elangbam M, Misra AK. Development of CAPS markers to identify Indian tea (Camellia sinensis) clones with high catechin content. 2016;15(2).

Lateef DD. DNA Marker Technologies in Plants and Applications for Crop Improvements. 2015;(May):7-18.

Grover A, Sharma PC, Grover A, Sharma PC. Critical Reviews in Biotechnology Development and use of molecular markers : past and present. 2016;8551. doi:10.3109/07388551.2014.959891.

Schulman AH. Molecular markers to assess genetic diversity. 2007;(September 2006):313-321. doi:10.1007/s10681-006-9282-5.

Kalendar R, Schulman AH. PROTOCOL IRAP and REMAP for retrotransposon-based genotyping and fingerprinting. 2007;1(5):2478-2484. doi:10.1038/nprot.2006.377.

Semagn K, Bjørnstad Å, Ndjiondjop MN. An overview of molecular marker methods for plants. 2006;5(25):2540-2568.

Appleby N, Edwards D, Batley J. Chapter 2 New Technologies for Ultra-High Throughput Genotyping in Plants. 513:19-39. doi:10.1007/978-1-59745-427-8.

Reddy MP, Sarla N, Siddiq EA. Inter simple sequence repeat ( ISSR ) polymorphism and its application in plant breeding. 2002:9-17.

Kumar LS. DNA markers in plant improvement : An overview. 1999;17:143-182.

Silva D, Santos G, Barroca M, Collins T. Chapter 5 Inverse PCR for Point Mutation Introduction. 1620:87-100. doi:10.1007/978-1-4939-7060-5.

K MK, Rs F, Ballani A, Vinita T, Yachana J. Potential and application of molecular markers techniques for plant genome analysis. 2014;2(1):169-188.

Tingey S V, Rafalski JA, Hanafey MK. Genetic Analysis with RAPD Markers. 2000;(1990).

Varshney RK, Graner A, Sorrells ME. Genic microsatellite markers in plants : features and applications. 2005;23(1). doi:10.1016/j.tibtech.2004.11.005.

Kalia RK, Rai MK, Kalia S. Microsatellite markers : an overview of the recent progress in plants. 2011:309-334. doi:10.1007/s10681-010-0286-9.

Profi M, Goulao LF, Oliveira CM. Chapter 11. 2014;1115:211-231. doi:10.1007/978-1-62703-767-9.