Plant transformation permits the introduction of the gene of interest for producing novel transgenic plants. "When a gene from one species is moved or relocated to another species by using recombinant DNA technology are called genetically modified organisms. Genetic engineering is one way to modify the plants by selecting for desired traits. Genetically modified organisms have foreign genes derive from not only plant source but also from bacteria, viruses, fungi, insects and animals. Transformation is the introduction and addition of the desired gene in plant for the generation of transgenic plant. Plant transformation is a challenging process for scientists. DNA transfer by artificial methods like DNA transfer through physical method is micro-injection, biolistic or gene gun methods, electroporation, silica carbide, microinjection, lipofection, microinjection. DNA also transfers by chemical methods. In natural method like in biological method, Agrobacterium-mediated transfer, Rhizobium, virus-mediated and planta transformation. Plant transformation involves three phases target gene, Plant tissues, vector for successful transformation. Plant transformation offers a momentous means to gain desire character or trait of interest. Plant transformation technique benefit agriculturalists to grow more crops in less area of land. And give more yield at less cost consumption. Plant transformation technology is familiarizing many crops with our desired characters. This review explains the natural method of plant transformation and benefits of transgenic plant.
Keywords: Gene expression, Transgenic, Particle bombardment, Agrobacterium tumefaciens-mediated Transformation, Origin of replication, Genetic engineering, Electro transformation, Hygromycin, Transgenic Crops.
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A New Issue was published – Volume 8, Issue 4, 2025
10-10-2025 11-07-2025