In the developing countries, Immuno Chromatographic Techniques (ICT) are used instead of ELISA which may give false positive and false negative results. But ELISA technique has more than 99% accuracy, sensitivity and specificity regarding the diagnosis of Hepatitis C Virus. A cross sectional study on 200 patients is the part of this study to check sensitivity of ELISA for HCV as well as to compare the sensitivity of ELISA and ICT method for HCV. For investigation of Pakistani patients using these techniques, 50 samples of control group (25 samples of negative control and 25 of positive control) and 150 samples of patients (suspected for HCV) were collected. Their HCV tests were first performed by using ICT kits. Then these samples were tested for anti-HCV antibody through ELISA by fully automated Instrument and their cut off values was determined. Results obtained by ELISA were compared by the results obtained by testing through ICT method. Results revealed that twenty-five patients suffering from hepatitis C were detected by both ELISA and ICT (positive control). Similarly, ELISA and ICT showed the negative results for all twenty-five negative control patients. Both ELISA and ICT showed the same results regarding detection of hepatitis C except two patients in experimental group. ICT showed positive result of a patient who was healthy and also confirmed negative by ELISA technique. Also this patient did not show any sign and symptom of hepatitis C. Further, ICT showed negative result of hepatitis C infected patient who has the sign and symptoms of hepatitis C and also ELISA showed positive result regarding detection of hepatitis C. It can be concluded from this experiment that both ELISA and ICT can be used for the detection of hepatitis C. However, the sensitivity of ELISA is higher as compared to ICT. Therefore, it can be recommended that ELISA is more appropriate method for the detection of hepatitis C.
Keywords: ELISA, Diagnosis, ICT, Antibody, Appropriate, Symptoms.
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Source of Funding:
This research was financially supported by Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan.
Competing Interests Statement:
The authors declare no competing financial, professional, or personal interests.
Consent for publication:
The authors declare that they consented to the publication of this research work.
Availability of data and material:
The authors are willing to share the data and material according to relevant needs.
Author’s Contributions:
Bushra Rashid, Muhammad Ishfaq and Rida Maqbool planned and designed the research; Bushra Rashid performed the experiments; Bushra Rashid, Rida Maqbool and Muhammad Ishfaq analysed the data; Bushra Rashid & Muhammad Ishfaq wrote the manuscript.
Acknowledgements:
Authors are thankful to Prof. Dr. Muhammad Babar and Muhammad Imran Qadir for reading and criticizing the draft of this manuscript.
