Urinary tract infections (UTIs) are among the most common bacterial infections worldwide and represent a significant public health concern affecting millions of individuals each year. This study aimed to isolate and identify uropathogens from urine samples and determine their antibiotic susceptibility patterns for effective treatment. Fourteen urine samples were collected from patients and analyzed using standard cultural, morphological, microscopic, and biochemical identification methods in the microbiology laboratory. Five major bacterial species were identified, with Escherichia coli and Staphylococcus aureus being the most prevalent and frequently isolated pathogens in the samples studied. Antibiotic susceptibility testing using the Kirby–Bauer disk diffusion method revealed that Gentamicin and Amikacin were the most effective antibiotics against the isolated uropathogens. The findings emphasize the importance of culture-based antibiotic prescription and routine susceptibility testing to prevent multidrug resistance and ensure proper clinical management of urinary tract infections. A urinary tract infection (UTI) is an infection that occurs in any part of the urinary system and is commonly caused by bacterial invasion. The urinary system includes the kidneys, ureters, bladder, and urethra, which work together to produce and eliminate urine from the body. Most infections primarily involve the lower urinary tract, particularly the bladder and the urethra, leading to symptoms such as burning sensation during urination and increased frequency of urination. Women generally have a higher risk of developing UTIs than men due to anatomical and physiological differences in the urinary tract structure. A urinary tract infection that affects the bladder can be painful, uncomfortable, and irritating for the patient. However, if the infection spreads to the kidneys, the condition can become serious and may lead to severe complications requiring immediate medical treatment.

Keywords: Urinary Tract Infection, Uropathogens, Antibiotic Resistance, Gram-Positive Bacteria, Uropathogens, Escherichia coli, Staphylococcus aureus, Antibiotic Susceptibility, Antimicrobial Resistance, Kirby–Bauer Method, Urine Culture, Gram-Negative Bacteria.

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Source of Funding:

This study did not receive any grant from funding agencies in the public, commercial, or not–for–profit sectors.

Competing Interests Statement:

Authors have declared no competing interests.

Consent for publication:

The authors declare that they consented to the publication of this study.

Institutional Review Board Statement:

Not applicable for this study.

Informed Consent:

Not applicable for this study.