Any nutritious substance that people or animals eat or drink or those plants absorb in order to maintain life and growth is called Food. With the huge increasing population of the world, food production from natural resources could not meet their needs. So researchers move to produce more food using molecular-level techniques. This type of food is called genetically modified food (GM food), whose genetic material has been altered which is not present already in nature. GM food is made to increase nutrient content by alternation, has many advantages for humans as it increases the nutritional content and formation of pest, drought, herbicide, and cold resistant plants. But at the same time, it has negative impacts also. It is genetically unsafe, causing organ damage and allergic reactions in the digestive tract. The researchers are trying to do their best to produce crops with their desired characteristics by using molecular-level techniques.

Keywords: Genetically modified organisms, Transformation, Food crops, Bacillus thuringiensis.

Dizon F, Costa S, Rock C, Harris A, Husk C, Mei J. Genetically Modified (GM) Foods and Ethical Eating. J Food Sci. 2016;81(2):R287-R291. doi:10.1111/1750-3841.13191.

Chaudhary G, Singh SK. Biotechnology Products in Everyday Life. Springer International Publishing; 2019. doi:10.1007/978-3-319-92399-4.

Verma C. A Review on Impacts of Genetically Modified Food on Human Health. Open Nutraceuticals J. 2011;4(1):3-11. doi:10.2174/1876396001104010003.

Mishra S, Singh RB, Dwivedi SP, et al. Effects of nutraceuticals on genetic expressions. Open Nutraceuticals J. 2009;2:70-80. doi:10.2174/1876396000902010070.

Zhang Y, Yu LC. Single-cell microinjection technology in cell biology. BioEssays. 2008;30(6):606-610. doi:10.1002/bies.20759.

Kim TK, Eberwine JH. Mammalian cell transfection : the present and the future. 2010:3173-3178. doi:10.1007/s00216-010-3821-6.

Birch RG. PLANT TRANSFORMATION: Problems and Strategies for Practical Application. Annu Rev Plant Physiol Plant Mol Biol. 1997;48(1):297-326. doi:10.1146/annurev.arplant.48.1.297.

Hefferon KL. Nutritionally enhanced food crops; progress and perspectives. Int J Mol Sci. 2015;16(2):3895-3914. doi:10.3390/ijms16023895.

Naseem M, Zaman MQ, Nazih H, et al. The effects of ginkgo biloba leaf extract on metabolic disturbances associated to alloxan-induced diabetic rats. J Anim Plant Sci. 2016;26(3):627-635. doi:10.1128/MMBR.67.1.16.

Dymek K, Rems L, Zorec B, Dejmek P, Galindo FG, Miklavčič D. Modeling electroporation of the non-treated and vacuum impregnated heterogeneous tissue of spinach leaves. Innov Food Sci Emerg Technol. 2015;29:55-64. doi:10.1016/j.ifset.2014.08.006.

Sharei A, Zoldan J, Adamo A, et al. A vector-free microfluidic platform for intracellular delivery. Proc Natl Acad Sci. 2013;110(6):2082-2087. doi:10.1073/pnas.1218705110.

Perrone S, Usai M, Lazzari P, Tucker SJ, Wallace HM, Zanda M. Efficient cell transfection with melamine-based gemini surfactants. Bioconjug Chem. 2013;24(2):176-187. doi:10.1021/bc3004292.

Batista Napotnik T, Miklavčič D. In vitro electroporation detection methods – An overview. Bioelectrochemistry. 2018;120:166-182. doi:10.1016/j.bioelechem.2017.12.005.

Kumar K, Karanwal S, Kumar Meena Chaudhary Charan Singh R, Rahul Kumar Meena Chaudhary Charan Singh C, Kumar Meena R, Jaiswal S. Role of biotechnology in crop and animal improvement for sustainable agriculture. J Pharmacogn Phytochem JPP. 2018;7(71):1120-1124.

Saito AC, Ogura T, Fujiwara K, Murata S, Nomura SIM. Introducing micrometer-sized artificial objects into live cells: A method for cell-giant unilamellar vesicle electrofusion. PLoS One. 2014;9(9):1-8. doi:10.1371/journal.pone.0106853.

Kumar B, Bidhan S, Krishi C, Sutradhar M, Chandra B, Viswavidyalaya K. Review Article GENETIC ENGINEERING FOR IMPARTING ABIOTIC STRESS TOLERANCE IN RICE -A REVIEW. 2016;(November).

Bennett WFD, Sapay N, Tieleman DP. Atomistic simulations of pore formation and closure in lipid bilayers. Biophys J. 2014;106(1):210-219. doi:10.1016/j.bpj.2013.11.4486.

Delalande A, Leduc C, Midoux P, Postema M, Pichon C. Efficient gene delivery by sonoporation is associated with microbubble entry into cells and the clathrin-dependent endocytosis pathway. Ultrasound Med Biol. 2015;41(7):1913-1926. doi:10.1016/j.ultrasmedbio.2015.03.010.

Meglic SH, Kotnik T. Electroporation-based applications in biotechnology. Handb Electroporation. 2017;3(8):2153-2169. doi:10.1007/978-3-319-32886-7_33.

Liu X, Fernandes R, Gertsenstein M, et al. Automated microinjection of recombinant BCL-X into mouse zygotes enhances embryo development. PLoS One. 2011;6(7):1-10. doi:10.1371/journal.pone.0021687.

Chow YT, Chen S, Wang R, et al. Single cell transfection through precise microinjection with quantitatively controlled injection volumes. Sci Rep. 2016;6(March):1-9. doi:10.1038/srep24127.

Ittner LM, Götz J. Pronuclear injection for the production of transgenic mice. Nat Protoc. 2007;2(5):1206-1215. doi:10.1038/nprot.2007.145.

Ikeda SR, Lovinger DM, McCool BA, Lewis DL. Heterologous expression of metabotropic glutamate receptors in adult rat sympathetic neurons: Subtype-specific coupling to ion channels. Neuron. 1995;14(5):1029-1038. doi:10.1016/0896-6273(95)90341-0.

Chow YT, Chen S, Liu C, et al. A high-throughput automated microinjection system for human cells with small size. IEEE/ASME Trans Mechatronics. 2016;21(2):838-850. doi:10.1109/TMECH.2015.2476362.

Chemicals R. Potent and specific.pdf. 1998;391(February):806-811. doi:10.1038/35888.

Sharan R, Gramm J, Yakhini Z, Ben-dor A. These authors contributed equally to this work. Relation. 2013;10(1.12):3377. doi:10.1128/MCB.00113-07.

Earth J. Chapter 2. 2006;1902(2010):1-39. doi:10.1029/2009JB006611.2-2.

TianZi C, ShenJie W, Jun Z, WangZhen G, TianZhen Z. Pistil drip following pollination: A simple in planta Agrobacterium-mediated transformation in cotton. Biotechnol Lett. 2010;32(4):547-555. doi:10.1007/s10529-009-0179-y.

Hashmi JA, Zafar Y, Arshad M, Mansoor S, Asad S. Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences. Virus Genes. 2011;42(2):286-296. doi:10.1007/s11262-011-0569-9.

Ahmad S. Genetic Transformation of Plants: Introduction and Recent Advances. Res J Pharmacogn Phytochem. 2017;9(2):125. doi:10.5958/0975-4385.2017.00023.1.

Firoozabady E, DeBoer DL, Merlo DJ, et al. Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants. Plant Mol Biol. 1987;10(2):105-116. doi:10.1007/BF00016148.

Umbeck P, Johnson G, Barton K, Swain W. Genetically Transformed Cotton (Gossypium Hirsutum L.) Plants. Bio/Technology. 1987;5(3):263-266. doi:10.1038/nm0798-822.

Fita A, Rodríguez-Burruezo A, Boscaiu M, Prohens J, Vicente O. Breeding and Domesticating Crops Adapted to Drought and Salinity: A New Paradigm for Increasing Food Production. Front Plant Sci. 2015;6(November):1-14. doi:10.3389/fpls.2015.00978.

Zhang C, Wohlhueter R, Zhang H. Genetically modified foods: A critical review of their promise and problems. Food Sci Hum Wellness. 2016;5(3):116-123. doi:10.1016/j.fshw.2016.04.002.

Puhan P. Advantages and Disadvantages of Popular Genetically Modified Plants and Animals-a Review. Eur J Pharm Med Res. 2018;5(2):175-182. www.ejpmr.com.

Articles R, Physician S, Program NR, Virology M, Diamond AL, Fellow D. Vitamin B-12 Associated Neurological Diseases. Contin Educ. 2006;(43):1-30. doi:10.1017/S0014479706343797.

Xiao Y, Wu K. Recent progress on the interaction between insects and Bacillus thuringiensis crops. 2019.

Moellenbeck DJ, Peters ML, Bing JW, et al. Nbt0701_668.Pdf. 2001;19(July).

Whitman DB. Genetically Modified Foods : Harmful or Helpful ? What are genetically-modified foods ? What are some of the advantages of GM foods ? CSA Discov Guid. 2001;(April 2000):1-13. doi:10.1111/tbed.12470.

Li FF, Wu SJ, Chen TZ, et al. Agrobacterium-mediated co-transformation of multiple genes in upland cotton. Plant Cell Tissue Organ Cult. 2009;97(3):225-235. doi:10.1007/s11240-009-9521-2.

Barrows G, Sexton S, Zilberman D. Agricultural Biotechnology: The Promise and Prospects of Genetically Modified Crops. J Econ Perspect. 2014;28(1):99-120. doi:10.1257/jep.28.1.99.

De Bruijn M, Verdonck-de Leeuw I, Ten Bosch L, et al. Phonetic-acoustic and feature analyses by a neural network to assess speech quality in patients treated for head and neck cancer. Proc Annu Conf Int Speech Commun Assoc INTERSPEECH. 2008:1753-1756. doi:10.1007/s10658-007-9229-2.

Cited R. United et States Patent. 2018;2.

Naegeli H, Birch AN, Casacuberta J, et al. Assessment of genetically modified cotton GHB614 × T304‐40 × GHB119 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 (application EFSA‐GMO‐NL‐2014‐122). EFSA J. 2018;16(7):1-30. doi:10.2903/j.efsa.2018.5349.

Wally O, Punja ZK. Genetic engineering for increasing fungal and bacterial disease resistance in crop plants. GM Crops. 2010;1(4):199-206. doi:10.4161/gmcr.1.4.13225.

Wani SH, Kumar V, Shriram V, Sah SK. Phytohormones and their metabolic engineering for abiotic stress tolerance in crop plants. Crop J. 2016;4(3):162-176. doi:10.1016/j.cj.2016.01.010.

Gürel F, Öztürk ZN, Uçarlı C, Rosellini D. Barley Genes as Tools to Confer Abiotic Stress Tolerance in Crops. Front Plant Sci. 2016;7(August). doi:10.3389/fpls.2016.01137.

Kreuze JF, Valkonen JP. Utilization of engineered resistance to viruses in crops of the developing world, with emphasis on sub-Saharan Africa. Curr Opin Virol. 2017;26(Cmd):90-97. doi:10.1016/j.coviro.2017.07.022.

Zaidi SS-A, Tashkandi M, Mansoor S, Mahfouz MM. Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance. Front Plant Sci. 2016;7(November):1-10. doi:10.3389/fpls.2016.01673.

Khatodia S, Bhatotia K, Tuteja N. Development of CRISPR / Cas9 mediated virus resistance in agriculturally important crops. 2017;5979. doi:10.1080/21655979.2017.1297347.

Mahmood A, Amaya R, Turgay OC, Yaprak AE, Taniguchi T, Kataoka R. High salt tolerant plant growth promoting rhizobacteria from the common ice-plant Mesembryanthemum crystallinum L. Rhizosphere. 2019;9(November 2018):10-17. doi:10.1016/j.rhisph.2018.10.004.

Ali A, Muzaffar A, Awan MMF, Din S, Nasir IA, Husnain T. Genetically Modified Foods: Engineered tomato with extra advantages. Adv Life Sci. 2014;(JANUARY):139-152.

Gerszberg A, Hnatuszko-Konka K. Tomato tolerance to abiotic stress: a review of most often engineered target sequences. Plant Growth Regul. 2017;83(2):175-198. doi:10.1007/s10725-017-0251-x.

Ramireddy E, Hosseini SA, Eggert K, et al. Root Engineering in Barley: Increasing Cytokinin Degradation Produces a Larger Root System, Mineral Enrichment in the Shoot and Improved Drought Tolerance. Plant Physiol. 2018;177(3):1078-1095. doi:10.1104/pp.18.00199.

Liu G, Gilding EK, Kerr ED, et al. Increasing protein content and digestibility in sorghum grain with a synthetic biology approach. J Cereal Sci. 2019;85(November 2018):27-34. doi:10.1016/j.jcs.2018.11.001.

Bagri DS, Upadhyay DC, Jain SK, Prakash C. Biotechnological improvement of nutritional and therapeutic value of cultivated potato 3 . PATHWAY ENGINEERING FOR ENHANCED AMINO ACID / PROTEIN. 2018;(1):217-228.

Garcia-Casal MN, Peña-Rosas JP, Giyose B, et al. Staple crops biofortified with increased vitamins and minerals: considerations for a public health strategy. Ann N Y Acad Sci. 2017;1390(1):3-13. doi:10.1111/nyas.13293.

Pons E, Alquézar B, Rodríguez A, et al. Metabolic engineering of β-carotene in orange fruit increases its in vivo antioxidant properties. Plant Biotechnol J. 2014;12(1):17-27. doi:10.1111/pbi.12112.

Welch RM, Graham RD. Breeding for micronutrients in staple food crops from a human nutrition perspective. J Exp Bot. 2004;55(396):353-364. doi:10.1093/jxb/erh064.

Blancquaert D, De Steur H, Gellynck X, Van Der Straeten D. Metabolic engineering of micronutrients in crop plants. Ann N Y Acad Sci. 2017;1390(1):59-73. doi:10.1111/nyas.13274.

Chauhan P, Mathur J. Potential of Helianthus annuus for phytoremediation of multiple pollutants in the environment : A Review. 2018;4:5-16.

Wu Z, Bañuelos GS, Lin Z-Q, et al. Biofortification and phytoremediation of selenium in China. Front Plant Sci. 2015;6(March):1-8. doi:10.3389/fpls.2015.00136.

Oh K, Cao T, Li T, Cheng H. Study on Application of Phytoremediation Technology in Management and Remediation of Contaminated Soils. J Clean Energy Technol. 2014;(201209026):216-220. doi:10.7763/JOCET.2014.V2.126.

Mishra S. Physiological and Biochemical Significance of Genetically Modified Foods: An Overview. Open Nutraceuticals J. 2013;6(1):18-26. doi:10.2174/1876396001306010018.

Jainuddinmull SM. An Overview of Genetically Modified Crops. J Pharmacogn Phytochem. 2018;7(1):2405-2410.

Mba JMS. Survey Reports Improved Health After Avoiding Genetically Modified Foods Part 2 : Survey Results Part 3 : Focus on Digestive Disorders. 2017.

Mathur R. Genetic Engineering and Biosafety in the use of Genetically Modified Foods. Int J Adv Sci Res Manag Spec Issue I. 2018;(I). www.ijasrm.com.

Smith EC, Taylor-Robinson AW. Parasite-specific immunoglobulin isotypes during lethal and non-lethal murine malaria infections. Parasitol Res. 2003;89(1):26-33. doi:10.1016/j.redox.2017.01.019.

Daily Mail T. Cancer row over GM foods as French study claims it did THIS to rats... and can cause organ damage and early death in humans. Dly Mail. 2012:1-11.

Lozano-Ojalvo D, Berin C, Tordesillas L. Immune basis of food allergic reactions. J Investig Allergol Clin Immunol. 2018;29(1):1-34. doi:10.18176/jiaci.0355.

Yavari B, Sarami S, Shahgaldi S, Athari SS, Sharma A. If there is really a Notable Concern about allergenicity of genetically modified foods? J Food Qual Hazards Control. 2016;3(1):3-9.

Remington B, Broekman HCH, Blom WM, et al. Approaches to assess IgE mediated allergy risks (sensitization and cross-reactivity) from new or modified dietary proteins. Food Chem Toxicol. 2018;112(December 2017):97-107. doi:10.1016/j.fct.2017.12.025.

Carzoli AK, Aboobucker SI, Sandall LL, Lübberstedt TT, Suza WP. Risks and opportunities of GM crops: Bt maize example. Glob Food Sec. 2018;19(October):84-91. doi:10.1016/j.gfs.2018.10.004