Dengue hemorrhagic fever and Dengue fever are harmful to human mostly children and they are a public health problem in Myanmar. An intervention study was conducted in Ywa-Thit village Hinthada Township as test village and Tha-Phan-Pin Ward Ingapu Township as non- intervention village. Six months study was conduct from May to October 2023 in Hinthada District Ayeyarwady Region. Fifty households each were randomly selected for Ae. aegypti larvae detection in water storage containers and Larval and Pupal Indices, Kye-containers and Key-premises were measured in both areas before and after interventions. Before intervention larva consuming rate of larvivorus fishes were tested against laboratory reared 3rd and 4th instar Aedes larvae in different water volumes. One inch length of Colisa labiosus found maximum number of larvae (1553±38) consumed within 24 hours was used to control Aedes larvae in water storage containers in Ywa-Thit village. In Ywa-Thit village 2 fishes each were put in major and one to two fish each was put in minor and miscellaneous containers. Result revealed that after intervention larva positive containers were significantly reduce from 64.19%, 35.16% and 66.23% to 0%, 0.89% and 1.61% respectively in major, Minor and miscellaneous containers in intervention area of Ywa-Thit village. Larval-Indices as Container-Index (CI), House-Index (HI) and Bretuae-Index (BI) were reduced from 51.75%, 78% and 296 to 0.76%, 2% and 4. Key-containers and Key-premises were also reduced from 24, 14 to 0, 0 respectively in Ywa-Thit village. Pupal-Index (Pupae/house, Pupae/container, Pupae/person and Pupae/child) were also reduced from 3.9, 0.68, 0.96 and 4.24 to 0 each in Ywa-Thit village (P=0.001). Although after-intervention, in nan-intervention Tha-Phan-Pin Ward, container positivity, larval and pupal indices, Key-containers and Key-premises were found to be increased and Key-containers and Key-premises were found increased to 62% and 34%. Native larvivorous fish Colisa labiosus is a very effective and suitable control tool for Aedes larvae in water storage containers in Hinthada Township. In conclusion, Colisa labiosus acts as a potential larvivorous effect of Aedes larvae and an effective biological control agent, eco-friendly, non-resistance and cost- effective, to be considered as a potent natural larvicidal agent to control Aedes larvae in the community.
Keywords: Aedes aegypti, Biological, Colisa labiosus, Consuming Rate, Containers, Fish, Larval Indices, Larvae, Larvivorous, Major, Minor, Miscellaneous, Pupal Indices, Water Storage Containers.
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Source of Funding:
This study received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.
Competing Interests Statement:
The authors declare that they have no competing interests related to this work.
Consent for publication:
The authors declare that they consented to the publication of this study.
Authors' contributions:
All the authors made an equal contribution in the Conception and design of the work, Data collection, Drafting the article, and Critical revision of the article. All the authors have read and approved the final copy of the manuscript.
Availability of data and materials:
Authors are willing to share data and material on request.
Institutional Review Board Statement:
Not applicable for this study.
Ethical Approval:
This study was approved by the ethical review committee of the Department of Medical Research, Myanmar.
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